Mesenchymal stromal cells alleviate depressive and anxiety-like …

Fig. 2. MSCs potentially exert an antidepressant

Fig. 2. MSCs potentially exert an antidepressant effect through 5-HT DRN neurons.

a Schematic diagram showing

a Schematic diagram showing the experimental procedures. b Representative images and quantification showing that 5-HT neurons in the DRN were eliminated by 5, 7-DHT injection. Scale bar, 100m. Two-tailed t test: t=15.43, df = 8, **** P=0.00000031. n=5 mice. 5, 7-DHT, 5,7-Dihydroxytryptamine. c Representative images and quantification showing that 5-HT neurons in the DRN were eliminated by AAV injection to overexpress Caspase3. Scale bar, 100m. Two-tailed t test: t=22.12, df = 8, **** P=0.000000018. n=5 mice. d, f Representative activity tracking in the OFT. e, g Statistical analysis of the total distance, the center distance, and the center time. e Total distance, one-way ANOVA: F(3, 36)=0.9807nsP=0.41. e Center distance, one-way ANOVA: F(3, 36)=7.363 *** P=0.00057. e Center time, one-way ANOVA: F(3, 36)=10.50 **** P=0.000042. g Total distance, Kruskal-Wallis test: ns P=0.46. g Center distance, Kruskal-Wallis test: ** P=0.0043. g Center time, one-way ANOVA: Treatment F(3, 36)=4.097 * P=0.013. n=10 mice. h, k Representative activity tracking in the EPM. i, l Statistical analysis of the time spent in and entry numbers to open arms. (i) Open arms time, Brown-Forsythe ANOVA test: *** P=0.00037. (i) Open arms entry number, one-way ANOVA: F(3, 36)=5.219 ** P=0.0043. l Open arms time, Kruskal-Wallis test: ** P=0.0030. l Open arms entry number, one-way ANOVA: F(3, 36)=5.485 ** P=0.0033. n=10 mice. j, m Statistical analysis of the immobile time in the TST. j Immobile time, one-way ANOVA: F(3, 36)=16.4 **** P=0.00000070. m Immobile time, one-way ANOVA: F(3, 36)=14.25 **** P=0.0000028. n=10 mice. Illustrations created with BioRender.com. Source data are provided as a Source Data file.

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Mesenchymal stromal cells alleviate depressive and anxiety-like ...