Human Pluripotent Stem Cells (iPSC) Generation, Culture, and …

1If FW of material is not 320.26, dilute appropriately to achieve a 10 mM solution. 2Work quickly; if the Matrigelis allowed to warm at all, it will become gel and will not be appropriate for plating. Matrigel cannot be thawed and refrozen. 3Warm complete medium required for that day at room temperature until it is no longer cool to the touch. Do not warm the medium at 37 C

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Induced pluripotent stem cell technology: a decade of progress

Nat Rev Drug Discov. Author manuscript; available in PMC 2019 Mar 14. Published in final edited form as: PMCID: PMC6416143 NIHMSID: NIHMS1013963 1Division of Stem Cell Biology Research, Department of Developmental and Stem Cell Biology, Beckman Research Institute of City of Hope, 1500 E. Duarte Rd, Duarte, CA 91010. 2Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho Shogoin Sakyo-ku Kyoto 606-8507, Japan

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